MOGAT2 Polyclonal antibody

MOGAT2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 19514-1-AP

Synonyms

DC5, DGAT2L5, hDC5, hMGAT2, MGAT2, MOGAT2



Tested Applications

Positive WB detected inmouse small intestine tissue, HEK-293 cells, rat small intestine
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 9 publications below

Product Information

19514-1-AP targets MOGAT2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name monoacylglycerol O-acyltransferase 2
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberNM_025098
Gene Symbol MOGAT2
Gene ID (NCBI) 80168
RRIDAB_10638917
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOGAT2, also named as DC5 and DGAT2L5, belongs to the diacylglycerol acyltransferase family. MOGAT2 catalyze the reaction: Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol. MOGAT2 has a preference toward monoacylglycerols containing unsaturated fatty acids. MOGAT2 plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. And it plays a role in diet-induced obesity. The antibody can recognize all these 3 isoform of MOGAT2.

Protocols

Product Specific Protocols
WB protocol for MOGAT2 antibody 19514-1-APDownload protocol
IP protocol for MOGAT2 antibody 19514-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Adipocyte iron levels impinge on a fat-gut crosstalk to regulate intestinal lipid absorption and mediate protection from obesity.

Authors - Zhuzhen Zhang
mouseWB

Nat Cell Biol

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Authors - Meng Shi
mouseWB

Cell Metab

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Authors - Hui Luo
humanWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
mouseWB

Nat Commun

Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.

Authors - Fangjun Yu
pigWB

Metabolites

Decreased Hyocholic Acid and Lysophosphatidylcholine Induce Elevated Blood Glucose in a Transgenic Porcine Model of Metabolic Disease

Authors - Jianping Xu