MOGAT2 Polyclonal antibody

MOGAT2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 19514-1-AP

Synonyms

DC5, DGAT2L5, hDC5, hMGAT2, MGAT2, MOGAT2

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Validation Data Gallery

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  WB analysis using 19514-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19514-1-AP (MOGAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19514-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19514-1-AP (MOGAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 19514-1-AP

  IP result of anti-MOGAT2 (IP:19514-1-AP, 4ug; Detection:19514-1-AP 1:300) with HEK-293 cells lysate 4800ug.

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Tested Applications

• Positive WB detected in: mouse small intestine tissue, HEK-293 cells, rat small intestine
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 9 publications below

Product Information

19514-1-AP targets MOGAT2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: monoacylglycerol O-acyltransferase 2
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 36-38 kDa
• GenBank Accession Number: NM_025098
• Gene Symbol: MOGAT2
• Gene ID (NCBI): 80168
• RRID: AB_10638917
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOGAT2, also named as DC5 and DGAT2L5, belongs to the diacylglycerol acyltransferase family. MOGAT2 catalyze the reaction: Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol. MOGAT2 has a preference toward monoacylglycerols containing unsaturated fatty acids. MOGAT2 plays a central role in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. And it plays a role in diet-induced obesity. The antibody can recognize all these 3 isoform of MOGAT2.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Adipocyte iron levels impinge on a fat-gut crosstalk to regulate intestinal lipid absorption and mediate protection from obesity.; Authors - Zhuzhen Zhang
mouse	WB	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
mouse	WB	Nat Commun; Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.; Authors - Fangjun Yu
pig	WB	Metabolites; Decreased Hyocholic Acid and Lysophosphatidylcholine Induce Elevated Blood Glucose in a Transgenic Porcine Model of Metabolic Disease; Authors - Jianping Xu