MOCS3 Polyclonal antibody

MOCS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27501-1-AP

Synonyms

MOCS3, UBA4

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Validation Data Gallery

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  WB analysis of MCF-7 using 27501-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27501-1-AP (MOCS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27501-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27501-1-AP (MOCS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 27501-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27501-1-AP (MOCS3 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 27501-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27501-1-AP (MOCS3 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 27501-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27501-1-AP (MOCS3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 27501-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27501-1-AP (MOCS3 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells
• Positive IHC detected in: human liver cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27501-1-AP targets MOCS3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOCS3 fusion protein Ag26596
• Full Name: molybdenum cofactor synthesis 3
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa, 65 kDa
• GenBank Accession Number: BC015939
• Gene Symbol: MOCS3
• Gene ID (NCBI): 27304
• RRID: AB_2880892
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOCS3, also named as molybdenum cofactor synthesis 3 and UBA4. The human MOCS3 protein contains an N-terminal domain similar to the Escherichia coli MoeB protein and a C-terminal segment displaying similarities to the sulfurtransferase rhodanese. MOCS3 is proposed to catalyze both the adenylation and the subsequent generation of a thiocarboxylate group at the C-terminus of the smaller subunit of molybdopterin (MPT) synthase during Moco biosynthesis in humans (PMID: 15910006). MOCS3 has a calculated molecular weight of 50 kDa and can be detected another band of 65 kDa due to urmylation (PMID: 21209336).