MOCS2B Polyclonal antibody

MOCS2B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14416-1-AP

Synonyms

MOCS2, MOCO1-B, MOCO1-A, MOCO1 A, MOCO1

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Validation Data Gallery

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  WB analysis using 14416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14416-1-AP (MOCS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14416-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14416-1-AP (MOCS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14416-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14416-1-AP (MOCS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14416-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14416-1-AP (MOCS2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of rat heart using 14416-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 14416-1-AP (MOCS2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 14416-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 14416-1-AP (MOCS2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14416-1-AP

  Immunofluorescent analysis of HeLa cells using 14416-1-AP (MOCS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, mouse heart tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14416-1-AP targets MOCS2B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOCS2B fusion protein Ag5761
• Full Name: molybdenum cofactor synthesis 2
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC046097
• Gene Symbol: MOCS2
• Gene ID (NCBI): 4338
• RRID: AB_2250779
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.