MOBKL3 Polyclonal antibody

MOBKL3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15886-1-AP

Synonyms

PHOCN, Mps one binder kinase activator-like 3, MOB4, CGI-95, 2C4D

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Validation Data Gallery

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  WB analysis of K-562 using 15886-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15886-1-AP (MOBKL3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 15886-1-AP

  IP result of anti-MOBKL3 (IP:15886-1-AP, 4ug; Detection:15886-1-AP 1:300) with K-562 cells lysate 4000ug.

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  IHC staining of human colon cancer using 15886-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15886-1-AP (MOBKL3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15886-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15886-1-AP (MOBKL3 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 15886-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15886-1-AP (MOBKL3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 15886-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15886-1-AP (MOBKL3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human colon cancer tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15886-1-AP targets MOBKL3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MOBKL3 fusion protein Ag8671
• Full Name: MOB1, Mps One Binder kinase activator-like 3 (yeast)
• Calculated Molecular Weight: 225 aa, 26 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC005237
• Gene Symbol: MOBKL3
• Gene ID (NCBI): 25843
• RRID: AB_10858478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.