MNDA Polyclonal antibody

MNDA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13576-1-AP

Synonyms

PYHIN3

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Validation Data Gallery

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  WB analysis of K-562 using 13576-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13576-1-AP (MNDA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human bladder using 13576-1-AP

  human bladder tissue were subjected to SDS PAGE followed by western blot with 13576-1-AP (MNDA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13576-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13576-1-AP (MNDA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 13576-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13576-1-AP (MNDA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, HL-60 cells, human bladder tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13576-1-AP targets MNDA in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MNDA fusion protein Ag4476
• Full Name: myeloid cell nuclear differentiation antigen
• Calculated Molecular Weight: 407 aa, 46 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC032319
• Gene Symbol: MNDA
• Gene ID (NCBI): 4332
• RRID: AB_2145071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Exp Mol Med; WISP-1 induced by mechanical stress contributes to fibrosis and hypertrophy of the ligamentum flavum through Hedgehog-Gli1 signaling.; Authors - Chao Sun