MMP-9 Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01003-2

  Sandwich ELISA standard curve of MP01003-2, Human MMP-9 Recombinant Matched Antibody Pair - PBS only. 82685-18-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0442. 82685-16-PBS was HRP conjugated as the detection antibody. Range: 39-2500 pg/mL.

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  Sample test of MP01003-2

  Serum of sixteen individual healthy human donors was measured. The MMP-9 concentration of detected samples was determined to be 213.0 ng/mL with a range of 67.5 - 426.4 ng/mL.

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  Sample test of MP01003-2

  Saliva of eight individual healthy human donors was measured. The MMP-9 concentration of detected samples was determined to be 80.7 ng/mL with a range of 1.9 - 260.9 ng/mL.

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  Sample test of MP01003-2

  Urine of eight individual healthy human donors was measured. The MMP-9 concentration of detected samples was determined to be 207.3 pg/mL with a range of 11.4 - 841.8 pg/mL.

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  Sample test of MP01003-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean MMP-9 concentration was determined to be 157.3 ng/mL in unstimulated PBMC supernatant, 237.9 ng/mL in PHA stimulated PBMC supernatant.

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  Cytometric bead array standard curve of MP01003-2

  Cytometric bead array standard curve of MP01003-2, MMP-9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82685-18-PBS. Detection antibody: 82685-16-PBS. Standard: Eg0442. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 82685-16-PBS

  Biolayer interferometry (BLl) kinetic assay of 82685-16-PBS against Human MMP-9 was performed. The affinity constant is 0.726 nM.

MMP-9 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 82685-16-PBS

Clone No.241224B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

MMP9, GELB, EC:3.4.24.35, CLG4B, 82 kDa matrix metalloproteinase-9

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Product Information

82685-16-PBS targets MMP-9 as part of a matched antibody pair:

MP01003-2: 82685-18-PBS capture and 82685-16-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• Calculated Molecular Weight: 707 aa, 78 kDa
• GenBank Accession Number: BC006093
• Gene Symbol: MMP-9
• Gene ID (NCBI): 4318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14780
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.