MMP8 Polyclonal antibody

• Featured Product
• KD/KO Validated

MMP8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 17874-1-AP

Synonyms

MMP 8, Matrix metalloproteinase-8, Matrix metalloproteinase 8, HNC, EC:3.4.24.34

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Validation Data Gallery

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  WB analysis of HepG2 using 17874-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17874-1-AP (MMP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17874-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17874-1-AP (MMP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17874-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17874-1-AP (MMP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17874-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP8 antibody (17874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, mouse liver tissue
• Positive IHC detected in: human placenta tissue, human kidney tissue, human ovary tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

17874-1-AP targets MMP8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MMP8 fusion protein Ag12367
• Full Name: matrix metallopeptidase 8 (neutrophil collagenase)
• Calculated Molecular Weight: 467 aa, 53 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC074988
• Gene Symbol: MMP8
• Gene ID (NCBI): 4317
• RRID: AB_2144582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487). Purified human neutrophil MMP-8 with proeMMP-8 at 70-75 KDa and active enzyme at 65 KDa and amniochorion proenzyme and active enzyme at 65 and 50 KDa, respectively(PMID: 28772283, PMID:15042023).

Publications

Species	Application	Title
mouse	WB	PLoS Pathog; Circumventing Y. pestis Virulence by Early Recruitment of Neutrophils to the Lungs during Pneumonic Plague.; Authors - Yaron Vagima
human,mouse	WB,IHC	Stem Cell Res Ther; Transplantation of human amniotic epithelial cells promotes morphological and functional regeneration in a rat uterine scar model.; Authors - Yihui Fan
human	WB,IHC	Am J Pathol; Preeclampsia is associated with alterations in DNA methylation of genes involved in collagen metabolism.; Authors - Mousa Ahmad A AA
mouse	IF	Viruses; Matrix Metalloproteinases Expression Is Associated with SARS-CoV-2-Induced Lung Pathology and Extracellular-Matrix Remodeling in K18-hACE2 Mice.; Authors - Hila Gutman
human	WB	Mediators Inflamm; Abnormal Expression of DICER1 Leads to Dysregulation of Inflammatory Effectors in Human Synoviocytes.; Authors - Congshan Jiang
rat	WB	Exp Eye Res; Luteolin ameliorates cornea stromal collagen degradation and inflammatory damage in rats with corneal alkali burn; Authors - Heng Wang