MMP2 Recombinant antibody, PBS Only

MMP2 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240131F5

Cat no : 81515-2-PBS

Synonyms

Gelatinase A, EC:3.4.24.24, CLG4A, 72 kDa type IV collagenase, 72 kDa gelatinase

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Validation Data Gallery

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  IF Staining of HepG2 using 81515-2-RR (same clone as 81515-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP2 antibody (81515-2-RR, Clone: 240131F5 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81515-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 81515-2-RR (same clone as 81515-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MMP2 Recombinant antibody (81515-2-RR, Clone:240131F5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81515-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HT-29 using 81515-2-RR (same clone as 81515-2-PBS)

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug MMP2 Recombinant Antibody (81515-2-RR, Clone:240131F5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81515-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81515-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81515-2-RR against Human MMP2 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 81515-2-PBS is MMP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MMP2 fusion protein Ag0549
• Full Name: matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 62-72 kDa
• GenBank Accession Number: BC002576
• Gene Symbol: MMP2
• Gene ID (NCBI): 4313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd). Western blot analysis showed that the 72 kDa and 62/65 kDa proteinase activities were pro-MMP2 and the active enzyme, respectively (PMID:11112697,PMID: 30867536).