Published Applications
IHC | See 1 publications below |
Product Information
66366-1-PBS targets MMP2 as part of a matched antibody pair:
MP51054-2: 66366-4-PBS capture and 66366-1-PBS detection (validated in Cytometric bead array)
MP51054-3: 66366-5-PBS capture and 66366-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | MMP2 fusion protein Ag25039 Predict reactive species |
Full Name | matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) |
Calculated Molecular Weight | 72 kDa |
Observed Molecular Weight | 55-74 kDa |
GenBank Accession Number | BC002576 |
Gene Symbol | MMP2 |
Gene ID (NCBI) | 4313 |
RRID | AB_2881746 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P08253 |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd).Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme,respectively(PMID:11112697).
Publications
Species | Application | Title |
---|---|---|
Int J Mol Sci Glial-Cell-Line-Derived Neurotrophic Factor Promotes Glioblastoma Cell Migration and Invasion via the SMAD2/3-SERPINE1-Signaling Axis |