MMP2 Monoclonal antibody, PBS Only (Detector)

• Featured Product
• KD/KO Validated

MMP2 Monoclonal Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2B10D1

Cat no : 66366-1-PBS

Synonyms

Gelatinase A, EC:3.4.24.24, CLG4A, 72 kDa type IV collagenase, 72 kDa gelatinase

---

Validation Data Gallery

• 

  WB analysis using 66366-1-Ig (same clone as 66366-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 66366-1-Ig (same clone as 66366-1-PBS)

  WB result of MMP2 antibody (66366-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP2 transfected U-251 cells. This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66366-1-Ig (same clone as 66366-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 66366-1-Ig (same clone as 66366-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66366-1-Ig (same clone as 66366-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66366-1-Ig (same clone as 66366-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66366-1-Ig (same clone as 66366-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66366-1-Ig (MMP2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66366-1-Ig (same clone as 66366-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66366-1-Ig (MMP2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66366-1-Ig (same clone as 66366-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66366-1-Ig (MMP2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66366-1-Ig (same clone as 66366-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug MMP2 Monoclonal antibody (66366-1-Ig, Clone:2B10D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-3 using 66366-1-Ig (same clone as 66366-1-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.4 ug MMP2 Monoclonal antibody (66366-1-Ig, Clone:2B10D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66366-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP51054-2

  Cytometric bead array standard curve of MP51054-2, MMP2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66366-4-PBS. Detection antibody: 66366-1-PBS. Standard:Ag25039. Range: 0.098-100 ng/mL.

---

Published Applications

• IHC: See 1 publications below

Product Information

66366-1-PBS targets MMP2 as part of a matched antibody pair:

MP51054-2: 66366-4-PBS capture and 66366-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MMP2 fusion protein Ag25039
• Full Name: matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 55-74 kDa
• GenBank Accession Number: BC002576
• Gene Symbol: MMP2
• Gene ID (NCBI): 4313
• RRID: AB_2881746
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd).Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme,respectively(PMID:11112697).

Publications

Species	Application	Title
human	IHC	Int J Mol Sci; Glial-Cell-Line-Derived Neurotrophic Factor Promotes Glioblastoma Cell Migration and Invasion via the SMAD2/3-SERPINE1-Signaling Axis; Authors - Xiaoxiao Guo