MMP12 Polyclonal antibody

MMP12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 22989-1-AP


HME, Macrophage elastase, Macrophage metalloelastase, Matrix metalloproteinase 12, ME, MME, MMP 12, MMP12

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22989-1-AP targets MMP12 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rabbit, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP12 fusion protein Ag19068
Full Name matrix metallopeptidase 12 (macrophage elastase)
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight 54 kDa, 45 kDa
GenBank Accession NumberBC112301
Gene Symbol MMP12
Gene ID (NCBI) 4321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MMP12(Matrix metalloproteinase-12) is a 54 kDa proenzyme that is processed into a 45 kDa and then a 22 kDa active form(15723202). MMP9 and MMP12 promote intimal thickening by independent cleavage of N-cadherin, which elevates vascular smooth muscle cell proliferation via beta-catenin signalling. Its overexpression in myeloid lineage cells plays a key role in modulating myelopoiesis, immune suppression, and lung tumorigenesis(PMID: 21378275).


Product Specific Protocols
WB protocol for MMP12 antibody 22989-1-APDownload protocol
IHC protocol for MMP12 antibody 22989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Cell Biol

Autophagy enables microglia to engage amyloid plaques and prevents microglial senescence

Authors - Insup Choi

Nat Commun

Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages.

Authors - Ludovic Boytard


MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.

Authors - Mingming Song

J Crohns Colitis

Matrix Metalloproteinase MMP-12 promotes macrophage transmigration across intestinal epithelial tight junctions and increases severity of experimental colitis.

Authors - Meghali Nighot

Redox Biol

Hydrogen sulfide attenuates cigarette smoke-induced airway remodeling by upregulating SIRT1 signaling pathway.

Authors - Ruijuan Guan

Respir Res

Role of PM2.5 in the development and progression of COPD and its mechanisms.

Authors - Junling Zhao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Hongxue (Verified Customer) (01-17-2023)

IHC staining in mouse liver, macrophage specific positive signaling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse liver
MMP12 Antibody Immunohistochemistry validation (1:500 dilution) in Mouse liver (Cat no:22989-1-AP)

Yadavalli (Verified Customer) (07-20-2021)

I had used the MMP12 antibody and detected in the lung tissue which ad shown a significant data

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Lung
MMP12 Antibody Western Blot validation (1:1000 dilution) in Lung (Cat no:22989-1-AP)

Hongxue (Verified Customer) (01-12-2021)

wb band very good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Liver
MMP12 Antibody Western Blot validation (1:1000 dilution) in Liver (Cat no:22989-1-AP)