Neprilysin/CD10 Recombinant antibody, PBS Only (Detector)

Neprilysin/CD10 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241322A8

Cat no : 84181-1-PBS

Synonyms

MME, Neprilysin, Enkephalinase, EC:3.4.24.11, CD10

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Validation Data Gallery

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  WB analysis of Raji using 84181-1-RR (same clone as 84181-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84181-1-PBS in a different storage buffer formulation.

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  WB analysis using 84181-1-RR (same clone as 84181-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84181-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84181-1-RR (same clone as 84181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84181-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84181-1-RR (same clone as 84181-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84181-1-RR (Neprilysin/CD10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84181-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 84181-1-RR (same clone as 84181-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Neprilysin/CD10 antibody (84181-1-RR, Clone: 241322A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84181-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01076-1

  Cytometric bead array standard curve of MP01076-1, MME,CD10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84181-3-PBS. Detection antibody: 84181-1-PBS. Standard: RP02332. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01076-3

  Cytometric bead array standard curve of MP01076-3, MME,CD10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84181-2-PBS. Detection antibody: 84181-1-PBS. Standard: RP02332. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84181-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84181-1-RR against Human Neprilysin/CD10 were performed. The affinity constant is 3.07 nM.

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Product Information

84181-1-PBS targets Neprilysin/CD10 as part of a matched antibody pair:

MP01076-1: 84181-3-PBS capture and 84181-1-PBS detection (validated in Cytometric bead array)

MP01076-3: 84181-2-PBS capture and 84181-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: membrane metallo-endopeptidase
• Calculated Molecular Weight: 750 aa, 86 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC101658
• Gene Symbol: CD10
• Gene ID (NCBI): 4311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).