MLL Polyclonal antibody

MLL Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 29278-1-AP

Synonyms

ALL 1, ALL1, CXXC7, FLJ11783, HRX, HTRX, HTRX1, KMT2A, Lysine N methyltransferase 2A, MLL, MLL/GAS7, MLL1, MLL1A, p180, p320 MLL cleavage product C180, TET1 MLL, Trithorax like protein, TRX1, Zinc finger protein HRX

---

Validation Data Gallery

• 

  WB analysis using 29278-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29278-1-AP (MLL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

29278-1-AP targets MLL in WB, IF, ChIP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MLL fusion protein Ag30468
• Full Name: myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)
• Calculated Molecular Weight: 432 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_001197104
• Gene Symbol: MLL
• Gene ID (NCBI): 4297
• RRID: AB_2918266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,ChIP	Nat Commun; KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics; Authors - Mai Abdel Mouti
human	IF	J Inflamm (Lond); Histone H3 posttranslational modified enzymes defined neutrophil plasticity and their vulnerability to IL-10 in the course of the inflammation; Authors - Paweł Piatek
human	WB	Cancer Lett; PARP1 interacts with WDR5 to enhance target gene recognition and facilitate tumorigenesis; Authors - Yali Qin