MLH3 Polyclonal antibody

• Featured Product
• KD/KO Validated

MLH3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25298-1-AP

Synonyms

HNPCC7, MLH3, mutL homolog 3 (E. coli), MutL protein homolog 3

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Validation Data Gallery

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  WB analysis of HepG2 using 25298-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25298-1-AP (MLH3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25298-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25298-1-AP (MLH3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 25298-1-AP is MLH3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MLH3 fusion protein Ag18201
• Full Name: mutL homolog 3 (E. coli)
• Calculated Molecular Weight: 1453 aa, 164 kDa
• Observed Molecular Weight: 164 kDa
• GenBank Accession Number: BC112167
• Gene Symbol: MLH3
• Gene ID (NCBI): 27030
• RRID: AB_2880015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB, IHC	Hum Mol Genet; Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.; Authors - Dazhang Bai ; KD Validated
mouse	WB	Prog Neurobiol; Loss of TDP-43 promotes somatic CAG repeat expansion in Huntington's disease knock-in mice; Authors - Dazhang Bai