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MLH1 Recombinant antibody, PBS Only (Capture)

MLH1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241472D1

Cat no : 84208-2-PBS

Synonyms

HNPCC2, HNPCC, hMLH1, FCC2, COCA2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MLH1 Recombinant antibody (84208-2-RR)

    WB analysis using 84208-2-RR (same clone as 84208-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84208-2-RR (MLH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MLH1 Recombinant antibody (84208-2-RR)

    IHC staining of human colon cancer using 84208-2-RR (same clone as 84208-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84208-2-RR (MLH1 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MLH1 Recombinant antibody (84208-2-RR)

    IF Staining of HeLa using 84208-2-RR (same clone as 84208-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MLH1 antibody (84208-2-RR, Clone: 241472D1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84208-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01140-1

    Cytometric bead array standard curve of MP01140-1

    Cytometric bead array standard curve of MP01140-1, MLH1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84208-2-PBS. Detection antibody: 84208-1-PBS. Standard: Ag2319. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84208-2-RR

    Affinity and Kinetic Characterization of 84208-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84208-2-RR against Human MLH1 were performed. The affinity constant is below 1 pM.

Product Information

84208-2-PBS targets MLH1 as part of a matched antibody pair:

MP01140-1: 84208-2-PBS capture and 84208-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MLH1 fusion protein Ag2319
Full Name mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
Calculated Molecular Weight 756 aa, 85 kDa
Observed Molecular Weight 85-100 kDa
GenBank Accession NumberBC006850
Gene Symbol MLH1
Gene ID (NCBI) 4292
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band.