MLH1 Polyclonal antibody

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  WB analysis of HeLa using 11697-1-AP

  WB result of MLH1 antibody (11697-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MLH1 transfected HeLa cells.

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  WB analysis of A431 using 11697-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11697-1-AP (MLH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11697-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11697-1-AP (MLH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11697-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11697-1-AP (MLH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11697-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11697-1-AP (MLH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11697-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11697-1-AP (MLH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11697-1-AP

  IP result of anti-MLH1 (IP:11697-1-AP, 4ug; Detection:11697-1-AP 1:1000) with HeLa cells lysate 1200 ug.

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  IF Staining of HeLa using 11697-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11697-1-AP (MLH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

MLH1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 11697-1-AP

Publications(32)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

MutL protein homolog 1, HNPCC2, HNPCC, hMLH1, FCC2

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, Jurkat cells, human testis tissue, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 13 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Product Information

11697-1-AP targets MLH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MLH1 fusion protein Ag2319
• Full Name: mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
• Calculated Molecular Weight: 756 aa, 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC006850
• Gene Symbol: MLH1
• Gene ID (NCBI): 4292
• RRID: AB_2145604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P40692
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MLH1, also named as COCA2, belongs to the DNA mismatch repair mutL/hexB family. It heterodimerizes with PMS2 to form MutL alpha which is a component of the post-replicative DNA mismatch repair system (MMR). MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. MLH1 also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. MLH1 heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.(PMID: 16873062, PMID: 18206974) Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS). Defects in MLH1 are a cause of Muir-Torre syndrome (MTS). Defects in MLH1 are a cause of susceptibility to endometrial cancer. Western blot analysis with an MLH1 antibody detected a 85-100 kDa band. Full-length human MLH1 is specifically cleaved into degradation products of 40-45 kDa by caspase-3 (PMID: 15087450, PMID: 19603033). This antibody is specific to MLH1.

Publications

Species	Application	Title
mouse	IHC	Cell Metab; Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer; Authors - Yu Jiang
mouse	IF	Nucleic Acids Res; NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.; Authors - Lu Chen
zebrafish	WB	Nucleic Acids Res; Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.; Authors - Jingzhen Li
human	IHC	J Hematol Oncol; Integrated proteogenomic characterization of urothelial carcinoma of the bladder.; Authors - Ning Xu
human	WB	Nucleic Acids Res; Selective tumor cell death induced by irradiated riboflavin through recognizing DNA G-T mismatch.; Authors - Yi Yuan
human	IHC	Cancer Lett; Inhibition of KIF20A enhances the immunotherapeutic effect of hepatocellular carcinoma by enhancing c-Myc ubiquitination; Authors - Shujia Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Manohar (Verified Customer) (12-18-2021)

No non-specific binding, blocked with milk for 1 hour and antibody incubation at 4degrees over night

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293