Melan-A Monoclonal antibody

Melan-A Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

4F10D6

Cat no : 60348-1-Ig

Synonyms

Antigen LB39 AA, Antigen SK29 AA, MART 1, MART1, melan A, Melan-A , MLANA, Protein Melan A

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Validation Data Gallery

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  IHC staining of human malignant melanoma using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (MLANA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human malignant melanoma using 60348-1-Ig

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (MLANA Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human malignant melanoma tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

60348-1-Ig targets Melan-A in IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Melan-A fusion protein Ag13387
• Full Name: melan-A
• Calculated Molecular Weight: 13 kDa
• GenBank Accession Number: BC014423
• Gene Symbol: MelanA
• Gene ID (NCBI): 2315
• RRID: AB_2881457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Melan-A is a melanocyte differentiation antigen, recognized by autologous cytotoxic T lymphocytes. Melan-A is also called MART-1 (melanoma antigen recognized by T cells). The Melan-A/MART-1 gene encodes this protein, 20-22 kDa, associated with endoplasmic reticulum and melanosomes. The function of the protein is unknown. Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas. Melan A is expressed in more than 85% of melanomas.

Publications

Species	Application	Title
human	IF	Cancer Commun (Lond); Candidate therapeutic agents in a newly established triple wild-type mucosal melanoma cell line.; Authors - Chaoji Shi