MKRN2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MKRN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12238-1-AP

Synonyms

RING-type E3 ubiquitin transferase makorin-2, RING finger protein 62, makorin ring finger protein 2, HSPC070, EC:2.3.2.27

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Validation Data Gallery

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  WB analysis using 12238-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12238-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12238-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 12238-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12238-1-AP (MKRN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12238-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12238-1-AP (MKRN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 12238-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MKRN2 antibody (12238-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 12238-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MKRN2 antibody (12238-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A375 cells, K-562 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below

Product Information

12238-1-AP targets MKRN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MKRN2 fusion protein Ag2881
• Full Name: makorin ring finger protein 2
• Calculated Molecular Weight: 416 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC015715
• Gene Symbol: MKRN2
• Gene ID (NCBI): 23609
• RRID: AB_2142636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MKRN2(Probable E3 ubiquitin-protein ligase makorin-2) is also named as RNF62. It is an E3 ubiquitin ligase that catalyzes the covalent attachment of ubiquitin moieties onto substrate proteins. It may be playing a functional role in negatively regulating neurogenesis via PI3K/Akt signaling.

Publications

Species	Application	Title
human	WB	Biochem Biophys Res Commun; Ubiquitination of IGF2BP3 by E3 ligase MKRN2 regulates the proliferation and migration of human neuroblastoma SHSY5Y cells.; Authors - Caiwei Jia ; KD Validated
human	WB	Oncol Lett; Ubiquitination of P53 by E3 ligase MKRN2 promotes melanoma cell proliferation.; Authors - Yiling Zhang ; KD Validated
human	WB	J Invest Dermatol; N6-methyladenosine modification of lnc-CCKAR-5 regulates autophagy in human umbilical cord mesenchymal stem cells by destabilizing LMNA and inhibits diabetic wound healing; Authors - Jian Wang