MKL1/MRTF-A Recombinant antibody

MKL1/MRTF-A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242021B4

Cat no : 84903-1-RR

Synonyms

MKL1, MRTFA, MKL/myocardin-like protein 1, MAL, KIAA1438

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Validation Data Gallery

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  WB analysis using 84903-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84903-1-RR (MKL1/MRTF-A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84903-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84903-1-RR against Human MKL1/MRTF-A were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84903-1-RR targets MKL1/MRTF-A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MKL1/MRTF-A fusion protein Ag15308
• Full Name: megakaryoblastic leukemia (translocation) 1
• Calculated Molecular Weight: 931 aa, 99 kDa
• Observed Molecular Weight: 145-160 kDa
• GenBank Accession Number: BC115039
• Gene Symbol: MKL1
• Gene ID (NCBI): 57591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Megakaryocytic leukemia 1 (MKL1), also known as myocardin-related transcription factor A (MRTF-A), is a transcriptional regulator involved in a wide range of pathophysiological processes in the cardiovascular system. MKL1 was initially identified as a co-factor for serum response factor (SRF) to regulate the transcription of contractile genes. Forced expression of MKL1 in non-muscle cells leads to robust up-regulation of contractile genes (PMID: 36587486, PMID: 37199168, PMID: 39222836). MKL1 is ubiquitously expressed and has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart, and brain. The molecular weight of MKL1 observed is consistent with what has been described in the literature (PMID: 34944031).