MIXL1 Polyclonal antibody

MIXL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22772-1-AP

Synonyms

MIX1 homeobox-like protein 1, Mix.1 homeobox-like protein, MIX, MILD1, Homeobox protein MIXL1



Tested Applications

Positive WB detected inHEK-293 cells, rat pancreas tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 6 publications below

Product Information

22772-1-AP targets MIXL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIXL1 fusion protein Ag18732
Full Name Mix1 homeobox-like 1 (Xenopus laevis)
Calculated Molecular Weight 232 aa, 25 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC113441
Gene Symbol MIXL1
Gene ID (NCBI) 83881
RRIDAB_2879164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mix1 homeobox-like 1 (MIXL1) Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression. This antibody is specific to MIXL1.

Protocols

Product Specific Protocols
WB protocol for MIXL1 antibody 22772-1-APDownload protocol
IF protocol for MIXL1 antibody 22772-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.

Authors - Jian Hui Low
humanIF

Adv Healthc Mater

Spatial Stem Cell Fate Engineering via Facile Morphogen Localization

Authors - Gyuhyung Jin

Cell Death Differ

Uric acid: a potent molecular contributor to pluripotent stem cell cardiac differentiation via mesoderm specification.

Authors - Bingbing Ke
humanIF

Int J Mol Sci

Porcine Kidney Organoids Derived from Naïve-like Embryonic Stem Cells

Authors - Meishuang Li
pigIF

Front Cell Dev Biol

Species origin of exogenous transcription factors affects the activation of endogenous pluripotency markers and signaling pathways of porcine induced pluripotent stem cells

Authors - Meng Zhou
humanIF

STAR Protoc

Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells.

Authors - Julie Bejoy