CCL20/MIP-3 alpha Polyclonal antibody

CCL20/MIP-3 alpha Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 26527-1-AP

Synonyms

CCL20, MIP-3 Alpha, MIP-3α, CCL-20, CCL 20

---

Validation Data Gallery

• 

  IHC staining of human lung cancer using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human tonsillitis using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26527-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26527-1-AP (MIP-3α Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive IHC detected in: human lung cancer tissue, human pancreas cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

26527-1-AP targets CCL20/MIP-3 alpha in IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCL20/MIP-3 alpha fusion protein Ag24069
• Full Name: chemokine (C-C motif) ligand 20
• Calculated Molecular Weight: 11 kDa
• GenBank Accession Number: BC020698
• Gene Symbol: CCL20/MIP-3 alpha
• Gene ID (NCBI): 6364
• RRID: AB_2880543
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.

Publications

Species	Application	Title
human	IF	Gut Microbes; Fusobacterium nucleatum promotes colorectal cancer metastasis through miR-1322/CCL20 axis and M2 polarization; Authors - Chaochao Xu
human	IF	Front Cell Dev Biol; EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma.; Authors - Bin Qi
mouse		Phytomedicine; The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides.; Authors - Gaofeng Cai
human	IHC	Oncol Rep; DEPDC1 drives hepatocellular carcinoma cell proliferation, invasion and angiogenesis by regulating the CCL20/CCR6 signaling pathway.; Authors - Wubin Guo
human	IHC	Inflammation; PMID: 31838663; Authors - YingYing Hu
human	IHC	Oncol Lett; Overexpression of B7-H4 promotes renal cell carcinoma progression by recruiting tumor-associated neutrophils via upregulation of CXCL8.; Authors - Anqi Li