MIP-1 alpha/CCL3 Polyclonal antibody

MIP-1 alpha/CCL3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27441-1-AP

Synonyms

C C motif chemokine 3, CCL 3, CCL3, CCL-3, chemokine (C C motif) ligand 3, G0S19 1, MIP 1 alpha, MIP 1 alpha/CCL3, MIP 1A, MIP-1 alpha, MIP1A, MIP-1α

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Validation Data Gallery

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  WB analysis using 27441-1-AP

  PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 27441-1-AP (MIP 1 alpha/CCL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27441-1-AP targets MIP-1 alpha/CCL3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MIP-1 alpha/CCL3 fusion protein Ag26764
• Full Name: chemokine (C-C motif) ligand 3
• Calculated Molecular Weight: 92 aa, 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC071834
• Gene Symbol: CCL3/MIP-1 alpha
• Gene ID (NCBI): 6348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1α, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.