MIOX Polyclonal antibody

MIOX Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17623-1-AP

Synonyms

Aldehyde reductase like 6, ALDRL6, Inositol oxygenase, Kidney specific protein 32, KSP32, MI oxygenase, MIOX, myo inositol oxygenase, Renal specific oxidoreductase, RSOR

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Validation Data Gallery

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  WB analysis using 17623-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17623-1-AP (MIOX antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 17623-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17623-1-AP (MIOX Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 17623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17623-1-AP (MIOX antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 17623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17623-1-AP (MIOX antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 17623-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 17623-1-AP (MIOX antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 17623-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 17623-1-AP (MIOX antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human kidney tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17623-1-AP is MIOX Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MIOX fusion protein Ag11829
• Full Name: myo-inositol oxygenase
• Calculated Molecular Weight: 285 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC073848
• Gene Symbol: MIOX
• Gene ID (NCBI): 55586
• RRID: AB_2878421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.