MINA Polyclonal antibody

• Featured Product
• KD/KO Validated

MINA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12214-1-AP

Synonyms

MDIG, MINA, Mina53, MYC induced nuclear antigen, NO52, Nucleolar protein 52, Ribosomal oxygenase 2, RIOX2, ROX

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 12214-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12214-1-AP (MINA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12214-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12214-1-AP (MINA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12214-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12214-1-AP (MINA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12214-1-AP (MINA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12214-1-AP

  IP result of anti-MINA (IP:12214-1-AP, 3ug; Detection:12214-1-AP 1:500) with HEK-293 cells lysate 2120ug.

• 

  IHC staining of human pancreas cancer using 12214-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12214-1-AP (MINA antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, mouse liver tissue, A375 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

12214-1-AP targets MINA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MINA fusion protein Ag2838
• Full Name: MYC induced nuclear antigen
• Calculated Molecular Weight: 465 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC014928
• Gene Symbol: MINA
• Gene ID (NCBI): 84864
• RRID: AB_2144191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MINA, also named as MDIG, MINA53 and NO52, belongs to the MINA53/NO66 family. It is involved in cellular proliferation. MINA may play an important role in cell growth and survival. It is involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. The transcriptional repressor MINA has been shown to repress Il4 gene expression by directly binding to its promoter, thereby, controlling TH2 bias.(PMID:21499227)

Publications

Species	Application	Title
mouse	WB,IF	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du ; KD Validated
mouse	WB	EMBO J; NFIL3/E4BP4 controls type 2 T helper cell cytokine expression.; Authors - Kashiwada Masaki M
human	WB	Cancer Res; ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.; Authors - Lili Zhang
human	WB	Oncol Lett; Effects of mdig on proliferation and apoptosis of lung cancer cells.; Authors - Xiaohui Xu ; KD Validated