MIDN Polyclonal antibody

• 

  WB analysis using 18939-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18939-1-AP (MIDN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 18939-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18939-1-AP (MIDN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 18939-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18939-1-AP (MIDN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

MIDN Polyclonal Antibody for WB, ELISA

Cat No. 18939-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

Midnolin

---

Tested Applications

• Positive WB detected in: PC-12 cells, BxPC-3 cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

18939-1-AP targets MIDN in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MIDN fusion protein Ag13492
• Full Name: midnolin
• Calculated Molecular Weight: 468 aa, 49 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC094778
• Gene Symbol: MIDN
• Gene ID (NCBI): 90007
• RRID: AB_2878569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q504T8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Midbrain nucleolar protein (midnolin, MIDN) is abundantly expressed in embryonic midbrain and localizes in the nucleus and nucleolus. MIDN mRNA was strongly and abundantly expressed during early developmental stages, and that the expression was not limited to midbrain but also expressed in diverse adult mouse tissues, including heart, spleen, lung, liver, skeletal muscle, kidney and testis.

Publications

Species	Application	Title
mouse	WB	Mol Cell Biol; CREB promotes beta cell gene expression by targeting Its coactivators to tissue-specific enhancers.; Authors - Sam Van de Velde
human	WB	Science; The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation; Authors - Xin Gu ; KO Validated
human	IHC,WB	Cancer Sci; Exosomal miR-222-3p contributes to castration-resistant prostate cancer by activating mTOR signaling; Authors - Weixi Wang