MID2 Monoclonal antibody, PBS Only

MID2 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9E4

Cat no : 68359-1-PBS

Synonyms

FLJ37715, FLJ41813, FXY2, MID2, Midin 2, midline 2, Midline defect 2, RING finger protein 60, RNF60, TRIM1

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Validation Data Gallery

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  WB analysis using 68359-1-Ig (same clone as 68359-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68359-1-Ig (MID2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68359-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 68359-1-Ig (same clone as 68359-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68359-1-Ig (MID2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68359-1-PBS in a different storage buffer formulation.

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Product Information

68359-1-PBS targets MID2 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MID2 fusion protein Ag30793
• Full Name: midline 2
• Calculated Molecular Weight: 715 aa, 81 kDa
• Observed Molecular Weight: 80~83 kDa
• GenBank Accession Number: BC017707
• Gene Symbol: MID2
• Gene ID (NCBI): 11043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Midline2 (MID2) is an ubiquitin-conjugating E2 enzyme that is involved in tumor development and has a physical interaction with breast cancer 1,early-onset (BRCA1).