MICAL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MICAL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

24

Reviews (3)

Cat no : 14818-1-AP

Synonyms

NICAL, NEDD9-interacting protein with calponin homology and LIM domains, MICAL, EC:1.14.13.225, [F-actin]-monooxygenase MICAL1

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Validation Data Gallery

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  WB analysis of HeLa using 14818-1-AP

  WB result of MICAL1 antibody (14818-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MICAL1 transfected HeLa cells.

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  WB analysis of Jurkat using 14818-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14818-1-AP (MICAL1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14818-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14818-1-AP (MICAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14818-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14818-1-AP (MICAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14818-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14818-1-AP (MICAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14818-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14818-1-AP (MICAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14818-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14818-1-AP (MICAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of T-47D using 14818-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 14818-1-AP (MICAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14818-1-AP

  IP result of anti-MICAL1 (IP:14818-1-AP, 5ug; Detection:14818-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IP experiment of T-47D using 14818-1-AP

  IP result of anti-MICAL1 (IP:14818-1-AP, 4ug; Detection:14818-1-AP 1:1000) with T-47D cells lysate 1800 ug.

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  IHC staining of human lung using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14818-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14818-1-AP (MICAL1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 14818-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MICAL1 antibody (14818-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of HeLa using 14818-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14818-1-AP (MICAL1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14818-1-AP

  IF result of MICAL1 antibody (14818-1-AP) with Hela Cells (Methanol fixation) MICAL1 in Red (endogenous) and BioGFP-MICAL1-FL overexpression. Courtesy of Qingyang Liu, Utrecht University.

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, T-47D cells, HeLa cells, human brain tissue, mouse lung tissue
• Positive IP detected in: HeLa cells, T-47D cells
• Positive IHC detected in: human lung tissue, human heart tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 15 publications below
• IHC: See 6 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

14818-1-AP targets MICAL1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MICAL1 fusion protein Ag6578
• Full Name: microtubule associated monoxygenase, calponin and LIM domain containing 1
• Calculated Molecular Weight: 118 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC052983
• Gene Symbol: MICAL1
• Gene ID (NCBI): 64780
• RRID: AB_2143754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions.There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL1 has four conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, a Lin11, Isl-1 and Mec-3 (LIM) domain and a C-terminal coiled-coil (CC) domain. MICAL1 is reported to regulate actin stress fibers and be required for normal actin organization. It may also be involved in apoptosis through binding with NDR (nuclear Dbf2-related) kinases. This antibody specially recognizes MICAL1.

Publications

Species	Application	Title
human	IF	Sci Adv; F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis.; Authors - Fengfeng Niu ; KO Validated
human	WB	Nat Commun; Oxidation of F-actin controls the terminal steps of cytokinesis.; Authors - Stéphane Frémont ; KD Validated
human	CoIP	Dev Cell; Amplification of F-Actin Disassembly and Cellular Repulsion by Growth Factor Signaling.; Authors - Jimok Yoon
human	WB	J Cell Biol; MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.; Authors - Chiara Galloni ; KD Validated
human	IHC	Oncogene; Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement; Authors - Ze Zhang
		Diabetes; Semaphorin3a promotes advanced diabetic nephropathy.; Authors - Pardeep K Aggarwal

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kincaid (Verified Customer) (06-02-2024)

Tested antibody in H4 cells. Diluted aMICAL1 1:500 in 3% BSA, diluted in TBS-tween (1%).

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: H4 cells

MICAL1 Antibody Western Blot validation (1:500 dilution) in H4 cells (Cat no:14818-1-AP)

FH

Christelle (Verified Customer) (11-21-2023)

For wb: used dilution 1/1000 For IP: used 2µg of antibody

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Megacaryocytic cell or humans platelets

MICAL1 Antibody Western Blot,Immunoprecipitation validation (1/1000 dilution) in Megacaryocytic cell or humans platelets (Cat no:14818-1-AP)

FH

Joleen (Verified Customer) (06-03-2019)

Shows band at where MICAL1 is expected but is faint. This image is a merge image of the membrane exposed for 120s and a stain free image to visualize the molecular weight. Even with long exposure, the band is faint.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

MICAL1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:14818-1-AP)