Anti-Human MICA/MICB (6D4) Mouse IgG2a Recombinant Antibody, PBS Only

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  FC experiment of HeLa using 65589-1-MR (same clone as 65589-1-PBS)

  1x10^6 HeLa cells were surface stained with 0.2 ug Anti-Human MICA/MICB Mouse IgG2a Recombinant Antibody (65589-1-MR, Clone: 6D4) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 65589-1-PBS in a different storage buffer formulation.

MICA/MICB Recombinant Antibody for FC

Cat No. 65589-1-PBS

Clone No.6D4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

MICA, MIC-A, MIC A, 6D4

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Product Information

65589-1-PBS targets MICA/MICB in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Transfected C1R cells expressing MICA
• Full Name: MHC class I polypeptide-related sequence A
• Calculated Molecular Weight: 383 aa, 43 kDa
• GenBank Accession Number: BC016929
• Gene Symbol: MICA
• Gene ID (NCBI): 100507436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.