MIB1 Recombinant antibody

MIB1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240676E4

Cat no : 83718-5-RR

Synonyms

DIP-1, DIP1, DIP 1, DAPK-interacting protein 1, DAPK interacting protein 1

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Validation Data Gallery

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  WB analysis using 83718-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83718-5-RR (MIB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83718-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MIB1 Recombinant antibody (83718-5-RR, Clone:240676E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of SH-SY5Y using 83718-5-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug MIB1 Recombinant antibody (83718-5-RR, Clone:240676E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83718-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83718-5-RR against Human MIB1 were performed. The affinity constant is 5.15 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, DU 145 cells, HEK-293 cells, K-562 cells, C6 cells, mouse brain tissue, rat brain tissue
• Positive FC (Intra) detected in: HeLa cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83718-5-RR is MIB1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MIB1 fusion protein Ag2507
• Full Name: mindbomb homolog 1 (Drosophila)
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC022403
• Gene Symbol: MIB1
• Gene ID (NCBI): 57534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MIB1(Mind bomb homolog 1) is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. MIB1, identified as an interactor of death associated protein kinase (DAPK), probably regulate apoptosis through ubiquitination and degradation of DAPK, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. MIB1 promotes proteasomal degradation of NRF2 and sensitizes lung cancer cells to ferroptosis(PMID: 34670864). In addition, MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation(PMID: 33793053).