MIB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MIB1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 11893-1-AP

Synonyms

DAPK interacting protein 1, DIP 1, DIP1, KIAA1323, MIB, MIB1, Mind bomb homolog 1, ZZANK2, ZZZ6

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Validation Data Gallery

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  WB analysis of HEK-293 using 11893-1-AP

  WB result of MIB1 antibody (11893-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MIB1 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 11893-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11893-1-AP (MIB1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11893-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11893-1-AP (MIB1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 11893-1-AP

  IP result of anti-MIB1 (IP:11893-1-AP, 4ug; Detection:11893-1-AP 1:500) with K-562 cells lysate 3400ug.

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  IHC staining of human kidney using 11893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11893-1-AP (MIB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11893-1-AP (MIB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue, HEK-293 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Product Information

11893-1-AP targets MIB1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MIB1 fusion protein Ag2507
• Full Name: mindbomb homolog 1 (Drosophila)
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC022403
• Gene Symbol: MIB1
• Gene ID (NCBI): 57534
• RRID: AB_2877803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MIB1(Mind bomb homolog 1) is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. MIB1, identified as an interactor of death associated protein kinase (DAPK), probably regulate apoptosis through ubiquitination and degradation of DAPK, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. MIB1 promotes proteasomal degradation of NRF2 and sensitizes lung cancer cells to ferroptosis(PMID: 34670864). In addition, MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation(PMID: 33793053).

Publications

Species	Application	Title
human,mouse	WB	Nat Commun; Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma; Authors - Weiwei Dai
human	WB	Cancer Res; Cancer-Specific Targeting of Taurine-Upregulated Gene 1 Enhances the Effects of Chemotherapy in Pancreatic Cancer.; Authors - Yoshihiko Tasaki
human	WB	Cancer Lett; LRP1 induces anti-PD-1 resistance by modulating the DLL4-NOTCH2-CCL2 axis and redirecting M2-like macrophage polarisation in bladder cancer; Authors - Hansen Lin
mouse	WB	Mol Med; Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis; Authors - Juan Xiao ; KD Validated
human	WB	Cell Death Dis; hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway.; Authors - Xinxing Wan
human	WB	MedComm (2020); Sanggenon C inhibits cell proliferation and induces apoptosis by regulating the MIB1/DAPK1 axis in glioblastoma; Authors - Hongbo Chang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xin (Verified Customer) (04-22-2021)

Good antibody, clear band seen in parental vs MIB1 knockout A549 cell

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549

MIB1 Antibody Western Blot validation (1:1000 dilution) in A549 (Cat no:11893-1-AP)