Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2)

MHC Class II (I-A/I-E) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse and More (1)

Applications

IF, FC

Conjugate

Unconjugated

Publications

2

CloneNo.

M5/114.15.2

Cat no : 65122-1-Ig

Synonyms

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 65122-1-Ig

  1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse MHC Class II (I-A/I-E) (65122-1-Ig, Clone: M5/114.15.2) and FITC anti-rat IgG2b Antibody at dilution 1:100 (red), or 0.5 ug Isotype Control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

65122-1-Ig targets MHC Class II (I-A/I-E) in IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Activated C57BL/6 mouse spleen cells
• Full Name: histocompatibility 2, class II antigen A, alpha
• GenBank Accession Number: BC031711
• Gene Symbol: MHC Class II
• Gene ID (NCBI): 14960
• RRID: AB_2918416
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

Publications

Species	Application	Title
mouse	IF	Int Immunopharmacol; Targeted inhibition of the immunoproteasome blocks endothelial MHC class II antigen presentation to CD4+ T cells in chronic liver injury; Authors - Yuwei Zhang
mouse	IF	J Ethnopharmacol; Jin-Gui-Shen-Qi Wan alleviates fibrosis in mouse diabetic nephropathy via MHC class II; Authors - Dan Liang