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MGLL Monoclonal antibody, PBS Only

MGLL Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A3E11

Cat no : 67846-1-PBS

Synonyms

HU K5, HUK5, Lysophospholipase homolog, Lysophospholipase like, MGL, MGLL, monoglyceride lipase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGLL Monoclonal antibody (67846-1-Ig)

    WB analysis using 67846-1-Ig (same clone as 67846-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67846-1-Ig (MGLL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67846-1-PBS in a different storage buffer formulation.

Product Information

67846-1-PBS targets MGLL in WB, Indirect ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MGLL fusion protein Ag7052
Full Name monoglyceride lipase
Calculated Molecular Weight 33 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC000551
Gene Symbol MGLL
Gene ID (NCBI) 11343
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain,there are two bands of 33 kDa and 35 kDa can be detected. The single protein species expressed in muscle is larger (around 40 kDa) (PMID:11470505). This protein can exsit as a homodimer (PMID:19957260).