MGEA5 Polyclonal antibody

• Featured Product
• KD/KO Validated

MGEA5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

77

Reviews (1)

Cat no : 14711-1-AP

Synonyms

OGA, Hexosaminidase C, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase

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Validation Data Gallery

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  WB analysis using 14711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14711-1-AP (MGEA5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14711-1-AP

  WB result of MGEA5 antibody (14711-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGEA5 transfected HepG2 cells.

• 

  WB analysis using 14711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14711-1-AP (MGEA5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14711-1-AP

  IP result of anti-MGEA5 (IP:14711-1-AP, 4ug; Detection:14711-1-AP 1:2000) with HepG2 cells lysate 1880 ug.

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  IHC staining of human pancreas using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human meningioma using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:50.

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  IHC staining of human meningioma using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 14711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGEA5 antibody (14711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 14711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGEA5 antibody (14711-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 14711-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MGEA5 (14711-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: SMMC-7721 cells, BxPC-3 cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human pancreas tissue, human meningioma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 64 publications below
• IHC: See 8 publications below
• IF: See 12 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Product Information

14711-1-AP targets MGEA5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, zebrafish, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MGEA5 fusion protein Ag6405
• Full Name: meningioma expressed antigen 5 (hyaluronidase)
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC039583
• Gene Symbol: MGEA5
• Gene ID (NCBI): 10724
• RRID: AB_2143063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present rabbit polyclonal antibody 14711-1-AP, which is consistent with results in a related reference (PubMed:11341771).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen
human	WB	J Clin Invest; Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation; Authors - Di Cui
human	IHC	Biomaterials; PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy; Authors - Lu Wang
rat	WB	Redox Biol; Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.; Authors - Wei Ou
mouse	WB	Proc Natl Acad Sci U S A; Polycomb repressive complex 2 is necessary for the normal site-specific O-GlcNAc distribution in mouse embryonic stem cells.; Authors - Myers Samuel A SA
Drosophila	WB	Proc Natl Acad Sci U S A; O-GlcNAc reports ambient temperature and confers heat resistance on ectotherm development.; Authors - Pablo T Radermacher

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (02-28-2019)

Always two bands together

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12

MGEA5 Antibody Western Blot, validation (1:1000 dilution) in C2C12 (Cat no:14711-1-AP)

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