MGAT4C Polyclonal antibody

• Featured Product
• KD/KO Validated

MGAT4C Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17841-1-AP

Synonyms

GlcNAc T IVc, GnT IVc, GNTIVH, HGNT IV H, MGAT4C

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Validation Data Gallery

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  WB analysis using 17841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17841-1-AP (MGAT4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17841-1-AP

  IP result of anti-MGAT4C (IP:17841-1-AP, 4ug; Detection:17841-1-AP 1:400) with mouse testis tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

17841-1-AP targets MGAT4C in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MGAT4C fusion protein Ag12201
• Full Name: mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative)
• Calculated Molecular Weight: 478 aa, 56 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC064141
• Gene Symbol: MGAT4C
• Gene ID (NCBI): 25834
• RRID: AB_2143048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MGAT4C is predicted to be a glycosyltransferase that catalyzes a GlcNAc β1-4 linkage to the core mannose residues of N-glycans, resulting in N-glycans with tri- or tetraantennary structures.Recently MGAT4C has been found to get involved in processing complex N-glycan structures on CD133 and CD133 has been identified as a substrate of MGAT4C. Given to the fact that CD133 is widely used as a marker of numerous stem cell and cancer stem cell types, MGAT4C may have an important role in certain cancers. (PMID: 21937449)

Publications

Species	Application	Title
mouse	IHC	Dev Cell; Wound-healing plasticity enables clonal expansion of founder progenitor cells in colitis; Authors - Cambrian Y Liu
human	WB	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB	J Biol Chem; CD133 protein N-glycosylation processing contributes to cell surface recognition of the primitive cell marker AC133 epitope.; Authors - Mak Anthony B AB ; KD Validated