MGAT4B Polyclonal antibody, PBS Only

MGAT4B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30718-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 30718-1-AP (same clone as 30718-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30718-1-AP (MGAT4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30718-1-AP (same clone as 30718-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30718-1-AP (MGAT4B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 30718-1-AP (same clone as 30718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 30718-1-AP (same clone as 30718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 30718-1-AP (same clone as 30718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 30718-1-AP (same clone as 30718-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MGAT4B antibody (30718-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30718-1-PBS in a different storage buffer formulation.

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Product Information

30718-1-PBS targets MGAT4B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MGAT4B fusion protein Ag33769
• Full Name: mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
• Calculated Molecular Weight: 548 aa, 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC009464
• Gene Symbol: MGAT4B
• Gene ID (NCBI): 11282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MGAT4B belongs to the N-Acetylglucosaminyltransferase-IV family and catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains (PMID: 17006639, 10372966). MGAT4B has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor to N-glycan biosynthesis (PMID:17006639).