MFSD2 Monoclonal antibody

MFSD2 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2D2B4

Cat no : 67965-1-Ig

Synonyms

MFSD2a, Major facilitator superfamily domain-containing protein 2A, HsMFSD2A, HMFN0656, FP1147

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Validation Data Gallery

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  WB analysis of human testis using 67965-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67965-1-Ig (MFSD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67965-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67965-1-Ig (MFSD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 67965-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 67965-1-Ig (MFSD2 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67965-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67965-1-Ig (MFSD2 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse brain using 67965-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFSD2 antibody (67965-1-Ig, Clone: 2D2B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue, human testis tissue
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67965-1-Ig targets MFSD2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MFSD2 fusion protein Ag31028
• Full Name: major facilitator superfamily domain containing 2
• Calculated Molecular Weight: 543 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC092414
• Gene Symbol: MFSD2
• Gene ID (NCBI): 84879
• RRID: AB_2918716
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MFSD2, also named as MFSD2A, belongs to a large family of presumptive carbohydrate transporters with 10-12 membrane-spanning domains, is located at chromosomal position 1p34.2, and is conserved in evolution. It plays a role in thermogenesis via beta-adrenergic signaling pathway. It may be the main plasma membrane tunicamycin transporter. MFSD2 has three isoforms with MW 50 kDa and 59-60 kDa.

Publications

Species	Application	Title
mouse	WB	Food Chem; Dietary sn-2 palmitate influences cognitive behavior by increasing the transport of liver-produced lysophosphatidylcholine VLCPUFAs to the brain; Authors - Teng Wei