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CoraLite® Plus 647-conjugated MFN1 Monoclonal antibody

MFN1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3F11C11

Cat no : CL647-66776

Synonyms

mitofusin 1, hfzo2, hfzo1, Fzo homolog, EC:3.6.5.-

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 647-conjugated MFN1 Monoclonal anti (CL647-66776)

    IF Staining of HepG2 using CL647-66776

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 MFN1 antibody (CL647-66776, Clone: 3F11C11 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated MFN1 Monoclonal anti (CL647-66776)

    FC experiment of HepG2 using CL647-66776

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human MFN1 (CL647-66776, Clone:3F11C11) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66776 targets MFN1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MFN1 fusion protein Ag4890
Full Name mitofusin 1
Calculated Molecular Weight 741 aa, 84 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC040557
Gene Symbol MFN1
Gene ID (NCBI) 55669
RRIDAB_2920289
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 MFN1 antibody CL647-66776Download protocol
FC protocol for CL Plus 647 MFN1 antibody CL647-66776Download protocol
Standard Protocols
Click here to view our Standard Protocols