MFN1 Monoclonal antibody, PBS Only

MFN1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3F11C11

Cat no : 66776-1-PBS

Synonyms

DKFZp762F247, FLJ20693, Fzo homolog, hfzo1, hfzo2, MFN1, mitofusin 1, Transmembrane GTPase MFN1

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Validation Data Gallery

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  WB analysis using 66776-1-Ig (same clone as 66776-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66776-1-Ig (same clone as 66776-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66776-1-Ig (same clone as 66776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66776-1-Ig (same clone as 66776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66776-1-Ig (same clone as 66776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66776-1-Ig (same clone as 66776-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66776-1-Ig (same clone as 66776-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN1 antibody (66776-1-Ig, Clone: 3F11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

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Product Information

66776-1-PBS targets MFN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MFN1 fusion protein Ag4890
• Full Name: mitofusin 1
• Calculated Molecular Weight: 741 aa, 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC040557
• Gene Symbol: MFN1
• Gene ID (NCBI): 55669
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).