MFG-E8 Polyclonal antibody

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  WB analysis using 25951-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25951-1-AP (MFG-E8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 25951-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 25951-1-AP (MFG-E8 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MFG-E8 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25951-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MFGE8, Lactadherin, hP47, HMFG, EDIL1

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Tested Applications

• Positive WB detected in: Jurkat cells, human placenta tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

25951-1-AP targets MFG-E8 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MFG-E8 fusion protein Ag13621
• Full Name: milk fat globule-EGF factor 8 protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC003610
• Gene Symbol: MFGE8
• Gene ID (NCBI): 4240
• RRID: AB_3669511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q08431
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Milk fat globule-epidermal growth factor-factor 8 (MFG-E8), also known as Lactadherin, is a multifunctional secreted glycoprotein exhibiting versatile functions in cell physiology affecting health and diseases. It plays roles in clearing apoptotic cells, anti-inflammatory, wound healing, arterial remodeling, angiogenesis, enhancing tumorigenicity, and cancer metastasis (PMID: 23972256). MFG-E8 consists of two-repeated EGF-like domains, a mucin-like domain, and two-repeated discoidin-like domains (C-domains), and contains an integrin-binding motif (RGD sequence) in the EGF-like domain (PMID: 11856354). It shows ubiquitous patterns of expression in various types of cells and tissues. Altered expression of MFG-E8 causes disruptions of homeostasis and is correlated with several diseases (PMID: 27429513).

Publications

Species	Application	Title
human	IHC	Heliyon; MFG-E8 promotes M2 polarization of macrophages and is associated with poor prognosis in patients with gastric cancer; Authors - Yang Li
human	WB	J Cell Mol Med; Linoleic Acid Enhances Renal Tubular Epithelial Cells Autophagy Caused by Calcium Oxalate Monohydrate Crystals; Authors - Weiming Ma