MEX3A Polyclonal antibody

• 

  WB analysis using 30865-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30865-1-AP (MEX3A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of SH-SY5Y using 30865-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using MEX3A antibody (30865-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

MEX3A Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30865-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RKHD4, MEX 3A

---

Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells, SKOV-3 cells, HCT 116 cells
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30865-1-AP targets MEX3A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEX3A fusion protein Ag33619
• Full Name: mex-3 homolog A (C. elegans)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: NM_001093725
• Gene Symbol: MEX3A
• Gene ID (NCBI): 92312
• RRID: AB_3086425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A1L020
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEX3A is a member of the MEX3 family, which widely exists in mammalian cells and encodes four different genes, MEX3A, MEX3B, MEX3C, and MEX3D. The MEX3 protein of mammals is composed of two RNA-binding KH domains and a ring domain with E3 ubiquitin ligase activity at the C terminal. The ring finger domain endows human MEX3 protein the ability to regulate ubiquitination of target proteins and then influencing their protein stability and subcellular localization.MEX3A plays complex and diverse roles in the development of various cancers. MEX3A plays a pivotal role in the post-transcriptional procession of mRNA and gene expression. MEX3A gene expression may be closely connected with malignant tumor development and progression. In most literature, MEX3A detects molecular weights between 60 and 70kDa (PMID:33159833,37845346,36354374,35490173,34976441,33159833)