METTL6 Polyclonal antibody

• Featured Product
• KD/KO Validated

METTL6 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16527-1-AP

Synonyms

tRNA N(3)-methylcytidine methyltransferase METTL6, Methyltransferase-like protein 6, methyltransferase like 6, hMETTL6, EC:2.1.1.-

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Validation Data Gallery

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  WB analysis using 16527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16527-1-AP (METTL6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16527-1-AP (METTL6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 16527-1-AP

  IP result of anti-METTL6 (IP:16527-1-AP, 4ug; Detection:16527-1-AP 1:5000) with MCF-7 cells lysate 1600 ug.

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  IHC staining of human lung cancer using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16527-1-AP (METTL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16527-1-AP (METTL6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16527-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16527-1-AP (METTL6 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue, human placenta tissue, human skin tissue, human brain tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

16527-1-AP targets METTL6 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: METTL6 fusion protein Ag9768
• Full Name: methyltransferase like 6
• Calculated Molecular Weight: 255 aa, 30 kDa
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC022400
• Gene Symbol: METTL6
• Gene ID (NCBI): 131965
• RRID: AB_2142190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent nontumor tissues. CRISPR/Cas9mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).

Publications

Species	Application	Title
human	IHC	Front Immunol; Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma; Authors - Yangying Zhou
human	WB	J Biol Chem; Three distinct 3-methylcytidine (m(3)C) methyltransferases modify tRNA and mRNA in mice and humans.; Authors - Luang Xu ; KO Validated
human	WB	Nat Commun; m3C32 tRNA modification controls serine codon-biased mRNA translation, cell cycle, and DNA-damage response; Authors - Jia Cui ; KO Validated