METTL4 Polyclonal antibody

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  IHC staining of human skin using 17718-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17718-1-AP (METTL4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17718-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17718-1-AP (METTL4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 17718-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17718-1-AP (METTL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

METTL4 Polyclonal Antibody for ELISA

Cat No. 17718-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

HsT661, methyltransferase like 4, METTL4

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Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

17718-1-AP targets METTL4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: METTL4 fusion protein Ag12093
• Full Name: methyltransferase like 4
• Calculated Molecular Weight: 472 aa, 54 kDa
• GenBank Accession Number: BC111020
• Gene Symbol: METTL4
• Gene ID (NCBI): 64863
• RRID: AB_2142047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N3J2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Methyltransferase like protein 4 (METTL4) is a internal m6Am methyltransferase, which mediates N6-methylation of Am30 on U2 snRNA in the context of an AAG motif in vivo and in vitro. METTL4 belongs to the MT-A70-like family, and like its paralogs METTL3/14, METTL4 is conserved from yeast to human.

Publications

Species	Application	Title
mouse	WB	EMBO J; PGE2 -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase.; Authors - Xixi Tao
human	WB	Am J Cancer Res; Binding of RNA m6A by IGF2BP3 triggers chemoresistance of HCT8 cells via upregulation of ABCB1.; Authors - Zhongmin Yang
human	IHC	Future Oncol; METTL4 and METTL5 as biomarkers for recurrence-free survival in hepatocellular carcinoma patients; Authors - Jialing Zhao