METTL3 Polyclonal antibody

METTL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Monkey

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29314-1-AP

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

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Validation Data Gallery

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  WB analysis using 29314-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29314-1-AP (METTL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29314-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29314-1-AP (METTL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 29314-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 29314-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 29314-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 29314-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, Neuro-2a cells, K-562 cells, MCF-7 cells, COS-7 cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human lung cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29314-1-AP targets METTL3 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Monkey samples.

• Tested Reactivity: Human, Mouse, Rat, Monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: METTL3 fusion protein Ag30638
• Full Name: methyltransferase like 3
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC001650
• Gene Symbol: METTL3
• Gene ID (NCBI): 56339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.