METTL14 Recombinant antibody, PBS Only

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  WB analysis using 80790-1-RR (same clone as 80790-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80790-1-RR (METTL14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 80790-1-RR (same clone as 80790-1-PBS)

  WB result of METTL14 antibody (80790-1-RR; 1:14000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL14 transfected HeLa cells. This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 80790-1-RR (same clone as 80790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80790-1-RR (METTL14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80790-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80790-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80790-1-RR against Human METTL14 were performed. The affinity constant is 9.12 nM.

• Featured Product
• KD/KO Validated

METTL14 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 80790-1-PBS

Clone No.2E7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

2E7, hMETTL14, Methyltransferase-like protein 14, METT L14, N6-adenosine-methyltransferase non-catalytic subunit

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Product Information

80790-1-PBS targets METTL14 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: METTL14 fusion protein Ag14325
• Full Name: methyltransferase like 14
• Calculated Molecular Weight: 456 aa, 52 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC007449
• Gene Symbol: METTL14
• Gene ID (NCBI): 57721
• RRID: AB_2918912
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9HCE5
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

METTL14, is also named as Methyltransferase-like protein 14 or KIAA1627, is a 456 amino acid protein, which belongs to the MT-A70-like family and localized in the nucleus. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naïve pluripotency-promoting transcripts results in transcript destabilization.