METTL1 Recombinant antibody, PBS Only

METTL1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241108G8

Cat no : 84272-1-PBS

Synonyms

Methyltransferase-like protein 1, methyltransferase like 1, EC:2.1.1.33, EC:2.1.1.-, C12orf1

---

Validation Data Gallery

• 

  WB analysis using 84272-1-RR (same clone as 84272-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84272-1-RR (METTL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84272-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 84272-1-RR (same clone as 84272-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug METTL1 Recombinant antibody (84272-1-RR, Clone:241108G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84272-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84272-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84272-1-RR against Human METTL1 were performed. The affinity constant is 0.526 nM.

---

Product Information

The immunogen of 84272-1-PBS is METTL1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: METTL1 fusion protein Ag7055
• Full Name: methyltransferase like 1
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31-35 kDa
• GenBank Accession Number: BC000550
• Gene Symbol: METTL1
• Gene ID (NCBI): 4234
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

METTL1 methyltransferase mediates m7G methylation within miRNAs and regulates cell migration via its catalytic activity. METTL1 can be inactivated by phosphorylation at Ser27 by protein kinase B (PKBα). Overexpression of METTL1 is widely observed among human cancers. It is also crucial for the regulation of chemoresistance in cancer treatment. In addition, mutations in the human N7-methylguanosine (m7G) methyltransferase complex METTL1/WDR4 cause primordial dwarfism and brain malformation.