MESDC2 Polyclonal antibody

MESDC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 10958-1-AP

Synonyms

MESDM, MESD, LRP chaperone MESD, LDLR chaperone MESD, KIAA0081

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Validation Data Gallery

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  WB analysis using 10958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10958-1-AP (MESDC2 antibody) at dilution of 1:800 incubated at 4 degree celsius over night.

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  WB analysis of HEK-293 using 10958-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10958-1-AP (MESDC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10958-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10958-1-AP (MESDC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 10958-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10958-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10958-1-AP

  Immunohistochemical analysis of paraffin-embedded normal stomach slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10958-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 10958-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, NIH/3T3 cells
• Positive IHC detected in: human placenta tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10958-1-AP targets MESDC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MESDC2 fusion protein Ag1403
• Full Name: mesoderm development candidate 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: BC009210
• Gene Symbol: MESDC2
• Gene ID (NCBI): 23184
• RRID: AB_2143722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (09-20-2023)

Works well in 293T cells

• Applications: Western Blot