MEPE Polyclonal antibody

MEPE Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23256-1-AP

Synonyms

MEPE, OF45, Osteoblast/osteocyte factor 45

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Validation Data Gallery

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  WB analysis of HeLa using 23256-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23256-1-AP (MEPE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 23256-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23256-1-AP (MEPE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23256-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23256-1-AP (MEPE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 23256-1-AP

  IP result of anti-MEPE (IP:23256-1-AP, 4ug; Detection:23256-1-AP 1:1000) with HeLa cells lysate 4000ug.

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  IHC staining of human lung using 23256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung slide using 23256-1-AP (MEPE Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 23256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung slide using 23256-1-AP (MEPE Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 23256-1-AP

  Immunofluorescent analysis of HepG2 cells, using MEPE antibody 23256-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23256-1-AP is MEPE Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEPE fusion protein Ag19758
• Full Name: matrix extracellular phosphoglycoprotein
• Calculated Molecular Weight: 525 aa, 58 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC128158
• Gene Symbol: MEPE
• Gene ID (NCBI): 56955
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases. (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a full-length human MEPE protein detects a major band of 98 kDa.