MELK Recombinant antibody, PBS Only

MELK Uni-rAb^TM Recombinant Antibody for Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230106D4

Cat no : 82893-1-PBS

Synonyms

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

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Validation Data Gallery

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  FC experiment of MCF-7 using 82893-1-RR (same clone as 82893-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human MELK (82893-1-RR, Clone:230106D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82893-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82893-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82893-1-RR against Human MELK were performed. The affinity constant is below 1 pM.

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Product Information

82893-1-PBS targets MELK in Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MELK fusion protein Ag1897
• Full Name: maternal embryonic leucine zipper kinase
• Calculated Molecular Weight: 651 aa, 75 kDa
• GenBank Accession Number: BC014039
• Gene Symbol: MELK
• Gene ID (NCBI): 9833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.