MELK Polyclonal antibody

MELK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11403-1-AP

Synonyms

hMELK, HPK38, KIAA0175, MELK, Protein kinase PK38

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Validation Data Gallery

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  WB analysis of Jurkat using 11403-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 11403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11403-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11403-1-AP (MELK Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11403-1-AP (MELK Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse colon using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, K-562 cells
• Positive IHC detected in: human breast cancer tissue, mouse colon tissue, mouse kidney tissue, human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

11403-1-AP targets MELK in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MELK fusion protein Ag1897
• Full Name: maternal embryonic leucine zipper kinase
• Calculated Molecular Weight: 651 aa, 75 kDa
• Observed Molecular Weight: 50-70 kDa
• GenBank Accession Number: BC014039
• Gene Symbol: MELK
• Gene ID (NCBI): 9833
• RRID: AB_2877762
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

Publications

Species	Application	Title
human	WB,IHC,IF,CoIP	Int J Oncol; MELK predicts poor prognosis and promotes metastasis in esophageal squamous cell carcinoma via activating the NF‑κB pathway.; Authors - Jiecheng Ye
human	IHC	Med Sci Monit; Up-Regulated Maternal Embryonic Leucine Zipper Kinase Predicts Poor Prognosis of Hepatocellular Carcinoma Patients in a Chinese Han Population.; Authors - Shaohan Wu
human	IHC	Drug Des Devel Ther; A Novel Tetramethylpyrazine Chalcone Hybrid- HCTMPPK, as a Potential Anti-Lung Cancer Agent by Downregulating MELK; Authors - Yan Ma
human	WB,IHC	J Cancer; Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma; Authors - Yan Wang