MEKK3 Polyclonal antibody

MEKK3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21072-1-AP

Synonyms

MAP3K3, MAPK/ERK kinase kinase 3, MAPKKK3, MEK kinase 3, MEKK 3, MEKK3

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Validation Data Gallery

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  WB analysis of mouse liver using 21072-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21072-1-AP (MEKK3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 21072-1-AP

  IP result of anti-MEKK3 (IP:21072-1-AP, 4ug; Detection:21072-1-AP 1:300) with mouse liver tissue lysate 7000ug.

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  IHC staining of human liver cancer using 21072-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21072-1-AP (MEKK3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 21072-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21072-1-AP (MEKK3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

21072-1-AP targets MEKK3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEKK3 fusion protein Ag15223
• Full Name: mitogen-activated protein kinase kinase kinase 3
• Calculated Molecular Weight: 657 aa, 74 kDa
• Observed Molecular Weight: 71 kDa, 74 kDa
• GenBank Accession Number: BC090859
• Gene Symbol: MEKK3
• Gene ID (NCBI): 4215
• RRID: AB_2878806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The mitogen-activated protein/ERK kinase kinase 3 (MEKK3) is a member of a family of MEKK/Ste11 serine/threonine protein kinases. One function of MEKK3 is to serve as an upstream regulatory kinase in the mitogen-activated protein kinase cascade, however, it also plays a critical role in other signaling pathways. MEKK3 regulates several important functions including cell survival and proliferation. It is ubiquitously expressed in multiple tissues and has been found in complexes with scaffold proteins and other kinases. (PMID: 18206350).Cerebral cavernous malformations (CCMs) complex regulation of MEKK3 is essential during vertebrate heart development. MEKK3 signalling and KLF2/4 may be targeted to develop new CCM therapeutics.

Publications

Species	Application	Title
human,mouse	WB,IF,IP	Oncogene; MEKK2 and MEKK3 suppress Hedgehog pathway-dependent medulloblastoma by inhibiting GLI1 function.; Authors - Jinqiu Lu
rat	WB	Sci Rep; Treadmill training improves lung function and inhibits alveolar cell apoptosis in spinal cord injured rats; Authors - Xianbin Wang