MEK1 Monoclonal antibody

• Featured Product
• KD/KO Validated

MEK1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3F6D7

Cat no : 67872-1-Ig

Synonyms

ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, MKK1, PRKMK1

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Validation Data Gallery

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  WB analysis of A431 using 67872-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (MEK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67872-1-Ig

  Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67872-1-Ig

  WB result of MEK1 antibody (67872-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEK1 transfected HeLa cells.

• 

  WB analysis of PC-3 using 67872-1-Ig

  Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67872-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67872-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67872-1-Ig (Phospho-MEK1 (Thr292) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, Calyculin A treated HeLa cells, NIH/3T3 cells, HSC-T6 cells, Calyculin A treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67872-1-Ig targets MEK1 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC139729
• Gene Symbol: MEK1
• Gene ID (NCBI): 5604
• RRID: AB_2918630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEK1 is also named as MAP2K1 (mitogen-activated protein kinase kinase 1), MAPKK1, PRKMK1, MKK1 and belongs to the MAP kinase kinase subfamily. It is a dual-specificity kinase that mediate ERK1 and ERK2 activation during adhesion and growth factor signaling(PMID:19219045). It also plays an essential role in extra-embryonic ectoderm during placentogenesis.

Publications

Species	Application	Title
rat	WB	J Ethnopharmacol; Jia-Wei-Si-Miao-Yong-An Fang stimulates the healing of acute radiation-induced cutaneous wounds through MAPK/ERK pathway; Authors - Yin Wang